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本课程起止时间为:2020-02-24到2020-06-30
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Lecture 1 Introduction of Gene Cloning and DNA Analysis (2020-02-25) GE test 1

1、 问题:In the laboratory, DNA molecules can be cut at specific sequences using:
选项:
A:UV light. 
B:restriction nucleases.
C:DNA ligase. 
D:laser tweezers.
E:DNA polymerase
答案: 【restriction nucleases.

2、 问题:You know that a strand of template DNA has the following sequence:5′-ATGGTGCCGGCCTGAAGTCA-3’Which of the following DNA fragments will complement the template DNA, according to Watson-Crick base-pairing rules?
选项:
A:3′-TAGGACCCGGGGACTTCAGT-5′
B:3′-ATGGTGCCGGCCTGAAGTCA-5′
C:3′-TACCACGGCCGGACTTCAGT-5′
D:3′-GAGGCACGGCCGGTCAACAGA-5′
答案: 【3′-TACCACGGCCGGACTTCAGT-5′

3、 问题:The two strands in the DNA double helix are anti-parallel. What does this mean?
选项:
A:Both strands run in the 3′ to 5′ direction
B:Both strands run in the 5′ to 3′ direction
C:Anti-parallel is used to refer to the left-handed curve of the DNA double helix
D:One strand runs in the 5′ to 3′ direction and the other runs 3 to 5 direction
答案: 【One strand runs in the 5′ to 3′ direction and the other runs 3 to 5 direction

4、 问题:In order to fit in the nucleus, DNA is highly organized. Which of the following structures is involved in packaging DNA?
选项:
A:Nucleosomes
B:Mitochondria
C:Golgi apparatus
D:Nucleoli
答案: 【Nucleosomes

5、 问题:Which of the following terms is used to describe the type of RNA that functions as an adaptor, linking amino acids with anticodons?
选项:
A:Messenger RNA (mRNA)
B:McroRNA( miRNA)
C:Ribosomal RNA (rRNA)
D:Transfer RNA (tRNA)
答案: 【Transfer RNA (tRNA)

6、 问题:During replication, which enzyme is responsible for making a daughter strand of DNA using the parent strand as template?
选项:
A:Reverse transcriptase
B:RNAse
C:DNA polymerase
D:RNA polymerase
答案: 【DNA polymerase

7、 问题:An electrical charge applied to an agarose gel will cause DNA to migrate through a matrix. What property of DNA does this technique exploit?
选项:
A:Electricity provides energy to nucleic acid,which causes the nucleic acid to move
B:The phosphate molecules that form the rails of the DNA ladder are negatively charged and will  migrate toward a positive charge
C:An electrical charge releases phosphate molecules from DNA,which provides energy that allows the DNA to migrate through the agarose gel
D:The nucleobases that form the rungs of the DNA ladder are positively charged and will migrate toward a negative charge
答案: 【The phosphate molecules that form the rails of the DNA ladder are negatively charged and will  migrate toward a positive charge

8、 问题:The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample?
选项:
A:To allow the primer to anneal to the template
B:To separate the two strands of DNA
C:To produce the optimal temperature for Taq dna polymerase to work
D:To produce the optimal temperature for most enzymatic reactions
答案: 【To separate the two strands of DNA

9、 问题:The first step in replication is separating the two strands of DNA. This task is achieved by the enzyme helicase, which unwinds the double stranded DNA. What does this create?
选项:
A:A replication fork
B:A transcription bubble
C:A DNA Zipper
D:Primase
答案: 【A replication fork

10、 问题:Which of the following is the best definition of “translation”?
选项:
A:Protein makes DNA
B:RNA makes DNA
C:RNA makes protein
D:DNA makes RNA
答案: 【RNA makes protein

11、 问题:Which of the following is a of the benefit of a semi-conservative replication process?
选项:
A:DNA can be quickly copied
B:This type of replication helps to minimizes mistakes
C:This type of replication requires fewer enzymes
D:There is no benefit
答案: 【This type of replication helps to minimizes mistakes

12、 问题:Which molecule(s) make up the rails of the DNA ladder? Select all that apply.
选项:
A:Single nucleobase
B:Paired nucleobases
C:Sugar molecules
D:Phosphate molecules
答案: 【Paired nucleobases;
Sugar molecules;
Phosphate molecules

13、 问题:Which of the following statement(s) describe(s) a difference between RNA and DNA. Select all that apply.
选项:
A:RNA can exit the nucleus
B:RNA is single stranded
C:RNA contains the nucleobase guanine(G)
D:RNA is less stable than dna
答案: 【RNA can exit the nucleus;
RNA is single stranded;
RNA is less stable than dna

Lecture 1 Introduction of Gene Cloning and DNA Analysis (2020-02-25) 转基因是什么?

1、 问题:现代杂交育种技术中建立的转基因智能不育系,育性是
选项:
A:完全可育的
B:完全不育的
C:部分不育的
D:不能确定
答案: 【部分不育的

2、 问题:A品系跟B品系杂交,是把A品系中几个基因转移到B细胞内?
选项:
A:1个
B:2个
C:多个
D:全套基因组基因
答案: 【全套基因组基因

3、 问题:基因修饰生物包括哪些类型?
选项:
A:抗虫转基因玉米
B:改变花色的转基因牵牛花
C:黄金大米
D:抗虫转基因棉花
答案: 【抗虫转基因玉米;
改变花色的转基因牵牛花;
黄金大米;
抗虫转基因棉花

4、 问题:转基因技术又叫做
选项:
A:基因工程技术
B:DNA重组技术
C:基因修饰技术
D:分子克隆技术
答案: 【基因工程技术;
DNA重组技术;
基因修饰技术;
分子克隆技术

5、 问题:基因工程第一步“切”的步骤包括切下目的基因,但不包括切开载体。
选项:
A:正确
B:错误
答案: 【错误

Lecture 2 Vectors for Gene Cloning (2020-02-28) 第三章 基因转移的运载车 章测题

1、 问题:细菌质粒载体所能携带的外源基因只限于()以下的DNA片段。
选项:
A:10kb
B:30kb
C:40kb
D:50kb
答案: 【10kb

2、 问题:柯斯质粒(cosmid)是一种 ()载体
选项:
A:容量最大的一种载体
B:由λ -DNA 的 cos 区与质粒重组而成的载体
C:是一种单链DNA环状载体
D:不能在受体细胞内复制,但可以表达的载体
答案: 【由λ -DNA 的 cos 区与质粒重组而成的载体

3、 问题:最早被用来动物细胞转基因的载体是
选项:
A:逆转录病毒
B:慢病毒
C:腺病毒
D:腺相关病毒
答案: 【逆转录病毒

4、 问题:基因工程载体应该具备那些特征?

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