2021 Molecular Biology(Wuhan University) 最新满分章节测试答案

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本课程起止时间为:2021-03-05到2021-06-25
本篇答案更新状态:已完结

【作业】1. Introduction Assignment-week 1

1、 问题:Please explain the experiment done by Frederick Griffith in 1928 which proved that DNA might be the key genetic material. Please try good academic expression.  
评分规则: 【 1. S cell was killed by heat treatment. (9)2. Killed S cell was mixed with R cell. (9)3. The mixture was cultured under proper condition. (9)4. R cell is transformed into S cell. (9)
】

2、 问题:Please give one example of a biological process at molecular level which shows the smartness of a living organism.
评分规则: 【 1. The example is a biological process. (10)2. It is interesting. (10)3. It is described at molecular level. (10) 
】

3、 问题:Please give an example of a biological process which involves forming and breaking of a weak bond under the physiological conditions present within cells.
评分规则: 【 1. The process involves forming and breaking of a bond. (12)2. The process is occurring in living cells. (10)3. The bond is a weak bond. (12)
】

【作业】2. Genome Structure, Chromatin, and the Nucleosome – I Assignment-week 2

1、 问题:Why are gene densities of eukaryotic genes lower than those of prokaryotic genes?
评分规则: 【 1. intergenic sequences are longer (10)2. individual genes are longer (6)(1) intron (2)(2) regulatory sequence (2)
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2、 问题:Please describe the composition of human genome.
评分规则: 【 1. about 3200 Mb (5)2. two parts: gene and gene related sequences, intergenic DNA (10)3. Intergenic DNA : about 2000 Mb, gene and gene related sequences: 1200 Mb (5)4. genes: 48 Mb, the rest: gene related sequences (5)5. Gene related sequences: introns, UTRs, gene fragment and pseudogenes (5)6. Intergenic sequences: genome wide repeats and other intergenic regions (5)7. Other intergenic regions: unique DNA, regulatory regions, miRNA and microsatellites. (5)
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3、 问题:Please describe the process of the generation of a pseudogene we talked in this week class. 
评分规则: 【 A functional gene is transcribed (10)Then, spliced (10)Reserves transcribed (10) Integrates into genome (10)
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【作业】3. Genome Structure, Chromatin, and the Nucleosome – II Assignment-week 3

1、 问题:Please describe the composition of a nucleosome.
评分规则: 【 1. Histone core (8)(1) H2A, H2B, H3, H4 (2)(2) Two copies (2)2. Core DNA (8)
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2、 问题:Pleas describe the process of nucleosome assembly.
评分规则: 【 1. H3 and H4 heterodimers (8)2. H3 and H4 tetramer (7)3. H3•H4 tetramer – DNA (8)4. two H2A•H2B dimers – H3•H4-DNA (7)
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3、 问题:What are the function of N-terminal tail of core histones?
评分规则: 【 1. Stabilize DNA wrapping around the histone octamer. (8)2. In the formation of the 30nm fiber. (8)3. The sites of extensive modifications that alter the function of individual nucleosomes. (8)
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4、 问题:Please describe the experiment of digestion of nucleosomal DNA with micrococcal nuclease we talked in this week class.  
评分规则: 【 1. light digestion of a DNA region with micrococcal nuclease (4)2. part of the light digestion products are subjected to gel electrophoresis after DNA extraction (4)3. the electrophoresis shows a ladder of DNA fragments of multiples of about 147 bp (5)4. part of the light digestion products are subjected to more extensive digestion (4)5. more extensive digestion products are subjected to gel electrophoresis after DNA extraction (4)6. the electrophoresis shows a band of DNA fragment of about 147 bp (5)
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【作业】4. Discussion Assignment-week 4

1、 问题:Please describe the steps of experiment in the following paper. Dintzis H.M. Assembly of the peptide chains of hemoglobin. Proc Natl Acad Sci USA. 1961 Jan 16; 47:247-261. 
评分规则: 【 Each step should be included. 
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【作业】5. The Structures of DNA and RNA Assignment-week 5

1、 问题:See attachment. 
评分规则: 【 I will do  it myself. 
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2、 问题:What is base flipping? How do you understand it biological relevance?
评分规则: 【 I will do it myself. 
】

3、 问题:You extracted plasmid A. You would like to know the approximate molecular mass of your plasmid by agarose gel electrophoresis. How would you do?
评分规则: 【 Ｉ　ｗｉｌｌ　ｄｏ　ｉｔ　ｍｙｓｅｌｆ．　
】

【作业】6. The Replication of DNA – I Assignment-week 6

1、 问题: What do you know about the chemistry of DNA synthesis after this week study?
评分规则: 【 I will do it myself. 
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2、 问题:What are Okazaki Fragments?
评分规则: 【 I will do it myself.